My project will investigate how CCNF regulates FOXM1 activation throughout the cell cycle by combining protein measurements with transcriptional snapshots and computational modeling. First, I’ll synchronize cells at key cycle phases and directly quantify Foxm1 and Cyclin F protein levels at each stage. Next, I’ll use RNA-seq data from those synchronized cultures—and from breast cancer samples—to infer stage-specific FOXM1 transcriptional activity. Finally, I’ll map those activity profiles onto a model to reveal how CCNF-driven timing shapes FOXM1 dynamics and downstream regulatory programs.